FTIR nanobiosensors for Escherichia coli detection
نویسندگان
چکیده
Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl)triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 10(2) CFU/mL, constituting a simple and selective method for the effective screening of water samples.
منابع مشابه
Detection of the eaeA Gene in Escherichia coli Isolated from Broiler Chickens by Polymerase Chain Reaction
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...
متن کاملBiofunctionalization of Gold Nanorods: A Comparative Study on Conjugation Methods for Fabrication of Nanobiosensors
Gold Nanorods have promised variety of applications in biomedicine and biosensing. As a fruitful candidate for early detection and imaging, these plasmonic nanoparticles have been utilized for diagnostic applications of interest. However, prior to design and fabricate SPR-based nanobiosensors, the type and nature of conjugation with biomolecules would be of utmost importance. Herein, four strat...
متن کاملPCR detection of Escherichia coli O157:H7 directed from slaughtered cattle in Shiraz, Iran
Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...
متن کاملDetection of Shiga toxin-producing Escherichia coli (STEC) in faeces of healthy calves in Mashhad, Iran
The aim of this study was to identify virulent Shiga toxin-producing Escherichia coli (STEC) strains isolated from faecal samples of 100 clinically healthy calves. In the present study, a total of 100 Escherichia coli (E. coli) isolates from clinically healthy calves belonging to 6 different farms located in Khorasan Razavi province, Iran, were examined for presence of virulence genes character...
متن کاملEvaluation of the antibiotic resistance and prevalence of uropathogenic Escherichia coli and detection of traT gene in isolated from patients referred to Abadan hospitals during 2017-2018
Objective: Escherichia coli is one of the most important causes agents of urinary tract infection in human. Thus, identification of Escherichia coli resistance patterns seems to be necessary. traT gene has been reported variable in Escherichia coli strains isolated from urinary tract infection. Therefore, the aim of this study was to investigate the prevalence of uropathogenic Escherichia coli ...
متن کامل